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. 2014 Jul 16;289(35):24383–24396. doi: 10.1074/jbc.M114.553883

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Dyn1 localizes with Mena during filopodia initiation and assembly. N1E-115 cells cultured on laminin-coated glass coverslips were transfected to express GFP-Mena (green) (panel i) and HA-Dyn1 (panel ii) and immunostained with anti-HA antibodies (red). A, HA-Dyn1 localized with bright puncta of GFP-Mena at the leading edge (arrowheads). Bar, 5 μm. Boxed areas in panels i–iii are shown enlarged as inserts 1–3. Bar,= 1 μm. B, HA-Dyn1 localized with GFP-Mena at filopodia tips (arrowheads) during filopodia assembly. Six filopodia were montaged to show the localization of Dyn relative to Mena. Bar, 5 μm.