TABLE 1.

Colocalization analysis of IRSp53 and Dyn in cells

N1E-115 cells were transfected to express Dyn-GFP and mRFP-IRSp53, fixed 24–28 h later, and acquired with confocal microscopy. Colocalization was determined using FluoView software (Olympus) and expressed as correlation coefficient (CC) ± S.D. (n = 5). A value of <0.25 indicates no colocalization, 0.25–0.5 indicates potential colocalization, 0.5–0.7 indicates partial colocalization, and >0.7 indicates high colocalization.

Sample (n = 5)	CC
	Cytosol	Lamellipodium	Neurite
Dyn1-GFP + mRFP-IRSp53	0.595 ± 0.142	0.819 ± 0.044	0.822 ± 0.045
Dyn2-GFP + mRFP-IRSp53	0.669 ± 0.153	0.915 ± 0.062	0.810 ± 0.061
Dyn3-GFP + mRFP-IRSp53	0.799 ± 0.048	0.876 ± 0.055	0.867 ± 0.075