TABLE 2.
Sample | Location | % FE | CC |
---|---|---|---|
Positive control (n = 6) | |||
RFP-GFP fusion | Cytosol | 17.8 ± 3.9 | −0.999 ± 0.001 |
Filopodium | 10.2 ± 2.1 | −0.885 ± 0.082 | |
Lamellipodium | 11.3 ± 4.9 | −0.860 ± 0.138 | |
Neurite | NA | NA | |
Negative control (n = 6) | |||
GFP + mRFP-IRSp53 | Cytosol | 2.79 ± 3.68 | −0.265 ± 0.531 |
Filopodium | 2.01 ± 3.87 | −0.028 ± 0.798 | |
Lamellipodium | 2.95 ± 3.64 | 0.003 ± 0.733 | |
Neurite | 3.26 ± 3.52 | −0.252 ± 0.653 | |
Sample (n = 9) | |||
Dyn1-GFP + mRFP-IRSp53 | Cytosol | 6.1 ± 0.6 | −0.833 ± 0.106 |
Filopodium | 10.8 ± 2.9 | −0.836 ± 0.123 | |
Lamellipodium | 12.8 ± 4.0 | −0.840 ± 0.114 | |
Neurite | 9.3 ± 4.0 | −0.864 ± 0.076 | |
Dyn1-GFP + mRFP-IRSp53FP/aa | Cytosol | 0.0 ± 2.3 | −0.190 ± 0.522 |
Filopodium | 1.3 ± 3.3 | 0.234 ± 0.340 | |
Lamellipodium | 1.0 ± 3.7 | −0.078 ± 0.616 | |
Neurite | 0.6 ± 4.6 | −0.033 ± 0.435 |