TABLE 2.

Dyn1 showed positive FRET with IRSp53

ROIs for AP-FRET experiments were chosen at cellular locations as follows: cytosol, filopodium, lamellipodium, and neurite. FRET efficiency and correlation coefficient (CC) values were calculated as described under “Experimental Procedures.” Positive FRET is defined as % FE >3% and Pearson's correlation coefficient values between −0.7 and −1.0. Data presented are averages ± S.D. from three experiments, with n = 6 or 9 per experiment. NA means not applicable.

Sample	Location	% FE	CC
Positive control (n = 6)
RFP-GFP fusion	Cytosol	17.8 ± 3.9	−0.999 ± 0.001
	Filopodium	10.2 ± 2.1	−0.885 ± 0.082
	Lamellipodium	11.3 ± 4.9	−0.860 ± 0.138
	Neurite	NA	NA
Negative control (n = 6)
GFP + mRFP-IRSp53	Cytosol	2.79 ± 3.68	−0.265 ± 0.531
	Filopodium	2.01 ± 3.87	−0.028 ± 0.798
	Lamellipodium	2.95 ± 3.64	0.003 ± 0.733
	Neurite	3.26 ± 3.52	−0.252 ± 0.653
Sample (n = 9)
Dyn1-GFP + mRFP-IRSp53	Cytosol	6.1 ± 0.6	−0.833 ± 0.106
	Filopodium	10.8 ± 2.9	−0.836 ± 0.123
	Lamellipodium	12.8 ± 4.0	−0.840 ± 0.114
	Neurite	9.3 ± 4.0	−0.864 ± 0.076
Dyn1-GFP + mRFP-IRSp53FP/aa	Cytosol	0.0 ± 2.3	−0.190 ± 0.522
	Filopodium	1.3 ± 3.3	0.234 ± 0.340
	Lamellipodium	1.0 ± 3.7	−0.078 ± 0.616
	Neurite	0.6 ± 4.6	−0.033 ± 0.435