TABLE 3.

Dyn1 is required for filopodia formation

N1E-115 cells, cultured in 35-mm laminin-coated glass-bottom dishes, were subjected to siRNA and then transfected with the cDNA constructs as shown. Differential interference contrast and fluorescence images of cells were acquired every 10 s at 24–32 h post-transfection. Time-lapse movies, generated from stack files with MetaMorph, were analyzed for cell morphology. Filopodia were defined as described under “Experimental Procedures,” and the numbers per cell were scored. Lifetime was measured by following filopodia from emergence to when it is no longer visible. Cells from three separate experiments were analyzed. Values were expressed as average ± S.D., (n = 10). Alphabets denote values with significant difference. * denotes values with significant difference as follows: *, p < 0.05; **, p < 0.01; ***, p < 0.001; #, filopodia with abnormal kinetics and increased lifetime were observed. Protrusions with lifetimes longer than 5 min were excluded from analysis.