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Non-reduced (A) and reduced (B) 15% SDS-PAGE of purified ASK1-TBD, TRX1, and their mixture (with 1:1 molar ratio) after the incubation in a buffer containing 20 mm Tris-HCl (pH 7.5), 200 mm NaCl, and 1 mm EDTA (and no reducing agents) for 0, 12, and 36 h at 4 °C.
