FIGURE 5.
Circular dichroism measurements. A, the comparison of the far-UV CD spectrum of the ASK41-TBD·TRX1 complex (solid line) with the sum of the individual far-UV CD spectra of ASK1-TBD and TRX1 (dotted line). B, the comparison of the near-UV CD spectrum of the ASK1-TBD·TRX1 complex (solid line) with the sum of the individual near-UV CD spectra of ASK1-TBD and TRX1 (dotted line). Proteins were mixed with the 1:1 molar stoichiometry. The mean residue ellipticity (MRE) is plotted as a function of the wavelength.