FIGURE 6.
Structural characterization of ASK1-TBD and its complex with reduced TRX1 by SAXS. A, scattering intensity as a function of the scattering vector s (s = 4π sin(θ)/λ, where 2θ is the scattering angle and λ is the wavelength). Inset shows Gunier plots of ASK1-TBD and the ASK1-TBD·TRX1 complex at concentrations 2.3 and 6 mg/ml, respectively. B, distance distribution function P(r). C, averaged and filtered DAMMIN shape envelope (spheres around the dummy residues) of ASK1-TBD. D, averaged and filtered DAMMIN shape envelope of the ASK1-TBD·TRX1 complex. The main difference between the envelope of the complex and those of ASK1-TBD alone is shown in red.