FIGURE 7.

Superposition of SAXS envelopes with the ab initio models of ASK1-TBD and the ASK1-TBD·TRX1 complex. A, superposition of the SAXS envelope with the theoretical model of ASK1-TBD (sequence 88–302) obtained by ab initio modeling using Robetta (34). The N-terminal CC motif of ASK1 is shown in dark red. Phe residues that were mutated to Trp are shown in green. Cys²⁵⁰ is shown in red. B, comparison of the calculated scattering curve of the theoretical model of ASK1-TBD (red line) with the experimental scattering data (black line). C, superposition of the SAXS envelope with the model of the ASK1-TBD·TRX1 complex that was created using the theoretical model of ASK1-TBD and the crystal structure of human TRX1 (37). The catalytic ³¹WCGPC³⁵ motif of TRX1 is shown in yellow. D, comparison of the calculated scattering curve of the theoretical model of the ASK1-TBD·TRX1 complex (red line) with the experimental scattering data (black line).