TABLE 1.

Summary of time-resolved fluorescence measurements of Trp³¹ of TRX1

Sample	τmeana,b	β1c,d	φ1c,e	β2c,d	φ2c,e	β3c,d	φ3c,e	βlongc,d	φlongc,e
	ns		ns		ns		ns		ns
TRX1 alone	1.62	0.018	<0.1	0.069	1.7			0.133	10
TRX1+ASK1-TBD WT	3.34					0.079	3.3	0.141	30
TRX1+ASK1-TBD C250S	4.52	0.026	<0.1	0.024	1.3	0.034	3.8	0.136	38

^a The mean fluorescence lifetime (τ_mean) was calculated using Equation 2.

^b S.D. = 0.05 ns.

^c The fluorescence anisotropies r(t) were analyzed for series of exponentials (Equation 3), where the amplitudes β_i represent the distribution of the correlation times φ_i. Initial anisotropy of Trp³¹ for all samples is r₀ = 0.22 ± 0.01. Amplitudes β₁ were calculated as β₁ = r₀ − (β₂+ β₃ + β_long) for each sample.

^d S.D. = 0.005.

^e S.D. = 15%.