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. 2014 Jul 20;289(35):24511–24520. doi: 10.1074/jbc.M114.575506

FIGURE 1.

FIGURE 1.

In situ analysis of the proximity of ABCD2-EGFP and ABCD1. Duolink proximity ligation assay between ABCD2-EGFP and ABCD1 or PMP22 in clone 28.38 (H4IIEC3 cells stably expressing ABCD2-EGFP upon doxycycline treatment) treated or not with doxycycline (Dox) (2 μg/ml) for 48 h was observed by confocal microscopy. The PLA signal demonstrates the proximity between ABCD2-EGFP and ABCD1. Negative control experiment shows the absence of PLA signal between ABCD2-EGFP and PMP22, an abundant peroxisomal membrane protein.