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Western blotting analysis of the expression of chimeric proteins in X-ALD fibroblasts cultivated at 37 or 30 °C. Nontransfected (−) or transfected X-ALD fibroblasts with pEGFP plasmids containing single or double cDNA sequences of ABCD1 and/or ABCD2 were cultivated at 37 °C (A) or 30 °C (B) during 68 h. Total cell proteins were solubilized and separated by SDS-PAGE followed by Western blot analysis with anti-GFP antibody. β-Tubulin was used as a loading control.
