FIGURE 10.

Thermal reversion rates for DrBphP PSM mutants affecting the hairpin/GAF domain contact. A, kinetics of Pfr → Pr thermal reversion for the mutant chromoproteins at 25 and 45 °C. B, half-life of Pfr for each mutant at 45 °C. Gray boxes represent the full-length (FL) chromoprotein and the PSM and PAS-GAF fragments. Green box represents the R202P mutant. Orange boxes represent various PHY-domain hairpin mutations. The dashed lines indicate the reversion rates for the PSM and PAS-GAF truncations. See Fig. 9 for the BV assembly and absorption spectra of each mutant.