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Subcellular location of METTL20 and METTL12. METTL20 or METTL12, each with C-terminal StrepII and FLAG tags were expressed in human 143B cells (upper and lower panels, respectively). A, cell nucleus stained with DAPI (blue). B, recombinant METTL20 or recombinant METTL12 detected with an anti-FLAG antibody, plus goat anti-mouse Alexa Fluor 488 (green). C, mitochondria stained with MitoTracker (red). D, merged areas of A–C.
