FIGURE 3.

Conformational fluctuations of HLA/VY8(P5A). a, chemical shift differences (Δω) between the major and minor states plotted on the crystal structure of HLA/VY8 as a continuous color scheme from gray to red. The positions around Tyr-99 and Ser-116, which are important for peptide recognition, are circled. b, relaxation dispersion profiles for Ser-116 and Tyr-99 recorded at 14.1 tesla (black) and 17.6 tesla (red). c, the van't Hoff plot for the fluctuations of pHLA between the major and minor states. The best-fit curve is shown as a solid line. The determinant factor (R²) is 0.99.