Table 1.

Methodological details, analyzed agents, and results of genotoxicity and/or DNA repair studies carried out in D. melanogaster with the comet assay.

Cell type	Treat. time (h)	%Agarose	Denaturat pH/time (min)	Electrophoresis V/cm/time (min)	Staining	Strain	Agents	Results	Reference
Brain cells	12	0.5	12.6/20	0.9/20	EthBr 40 μL (0.4 μg/mL)	OregonK, mus201	cDDP	+	García-Sar et al. (2008, 2012)
						OregonK, mus201, mus308,double mut	MMS	+	Rodríguez et al. (submitted)
			10–12.6/20			OregonK, mus201, mus308	MMS	+	Bilbao et al. (2002)
							EMS	+
							ENU	+
Hemocytes	24 ± 2	0.75	13/25	0.7/20	DAPI 20 μL (1 μg/mL)	OregonR	EMS	+	Carmona et al. (2011a)
							Cr(VI)-K2Cr2O7	+
							γ-rays	+
							PbCl2	-	Carmona et al. (2011b)
							Pb(NO3)2	+
							NiCl2	-	Carmona et al. (2011c)
							NiSO4	+
			13/20	0.73/25			AuNP	+	Sabella et al. (2011)
Mid-gut cells	74	0.75	> 13/10	0.7/15	EthBr 75 μL (20 μg/mL)/ 10 min	OregonR	Cypermethrin	+	Mukhopadhyay et al. (2004)
							Industrial waste leachates	+	Siddique et al. (2005b)
	24					OregonR	EMS	+	Siddique et al. (2005a)
							MMS	+
							ENU	+
							CP	+
						OregonR plus FPG and EndoIII enzimes	H2O2	+	Shukla et al. (2011)
							CdCl2	+
							CuSO4	+
	24/48					Transgenic (hsp70-lacZ)Bg	Graphene-Cu2O nanocomposite	+	Siddique et al. (2013)
	48					OregonR, mei9, mus201, mus210, mei41, mus207, mus209, mus309	Cr(III)-CrCl3	-	Mishra et al. (2011)
							Cr(VI)-K2Cr2O7	+
							Dichlorvos	+	Mishra et al. (2014)
	12–48					OregonR transgenic hsp70, hsp83, hsp26 plus FPG and EndoIII enzimes	Endosulfan	+	Sharma et al. (2012)
	48/72					OregonR, mei41, mus201, mus308, rad54	Industrial waste leachates	+	Siddique et al. (2008)
	48		8.5/60	14V-100mA/60		OregonR	CP	+	Sharma et al. (2011)
							BLM	+
							cDDP	+
							Cr(VI)	+
	48/24		8.5/60	14V-60mA/60		OregonR, ligiV, ku80, spn-A, okr, mre11	Cr(VI)-K2Cr2O7	+	Mishra et al. (2013)
Imaginal disk	48	0.75			Propidium iodide (1 μg/mL)	OregonR, Act-GAL4/CyO;+/+, Act-GAL4/Pol𝜖RNAi;+/+,+/+;pl10R/pl10R	BLM	+	Verma et al. (2012)
Cultured cells	6/24	0.5	Alkal/30	1/10	SYBR green	S2 cell line: standard and transfected	Paraquat	+	Radyuk et al. (2006)
							S-nitroso-N-acetyl penicillamine	+
	24/48	1	13/10	1/10	EthBr 40 μL (20 μg/mL)	S2 cell line	Methomil	+	Guanggang et al. (2013)