(A) Anterior segment preparation used for in vitro lens scanning; laser scans vertically from below, through the cornea (not shown) and lens; arrow shows posterior lens surface; ciliary processes also visible through the lens (arrowhead). (B) Scan showing the path of individual rays passing through a guinea pig cornea-lens complex before addition of carbachol to bath, with calculated BVD at each position superimposed (line with dots); 0 position on y-axis represents the optical center of the lens; (C, D) represent two examples obtained before, and 20 minutes after, the addition of 10 mM carbachol solution (• – baseline, □ – after carbachol).