Table.
Dilutions, Product Numbers, and Protocols Used for the Antibodies Used in This Study
Gene |
Company |
Product No. |
Fixation |
Blocking |
Secondary |
Dilution |
DRAQ5 | Biostatus Limited | DR50200 | A:M or 4% pfa in 1× PBS | BSA or goat serum | - | 1:2000 |
α-SMA | Sigma-Aldrich Corp. | F3777 | A:M or 4% pfa in 1× PBS | BSA or goat serum | FITC conjugated | 1:250 |
E-cadherin | Cell Signaling | 4065 | 4% pfa in 1× PBS | Goat serum | Anti-rabbit | 1:100 |
Aquaporin 0 | Millipore | ab3071 | A:M | 1% BSA | Anti-rabbit | 1:200 |
Pax6 | Millipore | AB2237 | 4% pfa in 1× PBS | Goat serum | Anti-rabbit | 1:200 |
c-Maf (M-153) | Santa Cruz Biotechnology | sc-7866 | A:M | 1% BSA | Anti-rabbit | 1:100 |
Prox1 | Belecky-Adams et al., 1997; Duncan et al., 2002 | A:M | 1% BSA | Anti-rabbit | 1:500 | |
Zeb1 (E-20) (δEF1) | Santa Cruz Biotechnology | sc-10572 | A:M | 1% BSA | Anti-goat | 1:200 |
Sip1 (H-260) | Santa Cruz Biotechnology | sc-48789 | 4% pfa in 1× PBS | Goat serum | Anti-rabbit | 1:100 |
Keratin 8 | Developmental Hybridoma Bank | Troma-1 | A:M | 1% BSA | Anti-rat | 1:100 |
A:M, 1:1 acetone:methanol solution; pfa, paraformaldehyde.