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. 2014 Aug 28;55(8):5445–5455. doi: 10.1167/iovs.14-14845

Table.

Dilutions, Product Numbers, and Protocols Used for the Antibodies Used in This Study

Gene
Company
Product No.
Fixation
Blocking
Secondary
Dilution
DRAQ5 Biostatus Limited DR50200 A:M or 4% pfa in 1× PBS BSA or goat serum - 1:2000
α-SMA Sigma-Aldrich Corp. F3777 A:M or 4% pfa in 1× PBS BSA or goat serum FITC conjugated 1:250
E-cadherin Cell Signaling 4065 4% pfa in 1× PBS Goat serum Anti-rabbit 1:100
Aquaporin 0 Millipore ab3071 A:M 1% BSA Anti-rabbit 1:200
Pax6 Millipore AB2237 4% pfa in 1× PBS Goat serum Anti-rabbit 1:200
c-Maf (M-153) Santa Cruz Biotechnology sc-7866 A:M 1% BSA Anti-rabbit 1:100
Prox1 Belecky-Adams et al., 1997; Duncan et al., 2002 A:M 1% BSA Anti-rabbit 1:500
Zeb1 (E-20) (δEF1) Santa Cruz Biotechnology sc-10572 A:M 1% BSA Anti-goat 1:200
Sip1 (H-260) Santa Cruz Biotechnology sc-48789 4% pfa in 1× PBS Goat serum Anti-rabbit 1:100
Keratin 8 Developmental Hybridoma Bank Troma-1 A:M 1% BSA Anti-rat 1:100

A:M, 1:1 acetone:methanol solution; pfa, paraformaldehyde.