Table 1. Classification and general features of A. variabilis ATCC 9413 according to the MIGS recommendations [14].

MIGS ID	Property	Term	Evidence Code
	Current Classification	Domain Bacteria	TAS [15]
		Phylum Cyanobacteria
		Class Nostocophycidae
		Order Nostocales
		Family Nostocaceae
		Genus Anabaena	TAS [16]
		Species Anabaena variabilis
	Gram stain	Negative	TAS [17]
	Cell shape	Ovoid cells in filaments	TAS [18]
	Motility	Hormogonia	TAS [19]
	Sporulation	Akinetes	TAS [20]
	Temperature range	20 – 40°C	TAS [21]
	Optimum temperature	35°C	TAS [22]
MIGS-22	Relationship to Oxygen	Aerobic	NAS
	Carbon source	Autotroph, heterotroph	TAS [23]
	Energy source	Phototroph, heterotroph	TAS [23]
MIGS-6	Habitat (EnvO)	Fresh water	TAS [2]
MIGS-6.3	Salinity	1.5% (maximum)	TAS [24]
MIGS-10	Extrachromosomal elements	4	TAS (this report)
MIGS-11	Estimated Size	7.1 Mb	TAS (this report)
MIGS-14	Known Pathogenicity	None	NAS
MIGS-15	Biotic Relationship	Free living; symbiotic	IED
MIGS-4	Geographic Location	Isolated Mississippi, 1964	TAS [2]
MIGS-4.1	Latitude	Not reported
MIGS-4.2	Longitude	Not reported
MIGS-4.3	Depth	Not reported
MIGS-4.4	Altitude	Not reported

Evidence codes - IDA: Inferred from Direct Assay (first time in publication); TAS: Traceable Author Statement (i.e., a direct report exists in the literature); NAS: Non-traceable Author Statement (i.e., not directly observed for the living, isolated sample but based on a generally accepted property for the species, or anecdotal evidence). These evidence codes are from the Gene Ontology project [25]. If the evidence code is IDA, then the property was directly observed for a living isolate by one of the authors or an expert mentioned in the acknowledgements.