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. 2014 Aug 24;11:150. doi: 10.1186/1743-422X-11-150

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© Saiyasombat et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of the replication kinetics of MODV-WNV(prM-E), MODV and WNV in Vero cells. Subconfluent monolayers of Vero cells were inoculated with MODV-WNV(prM-E), MODV and WNV at a m.o.i of 0.1. Supernatants were collected daily for 7 days and tested by plaque assay. MODV-WNV(prM-E) had been passaged once in BHK-21 cells and once in Vero cells prior to the experiments.