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. Author manuscript; available in PMC: 2014 Aug 29.
Published in final edited form as: Cell. 2007 Apr 6;129(1):195–206. doi: 10.1016/j.cell.2007.01.050

Figure 6. Intrahippocampal injection of Sal003 impairs contextual memory.

Figure 6

A) Western blots from hippocampal tissue show decreased phosphorylation of eIF2α after contextual fear conditioning. Dorsal hippocampi of rats were removed at different times after training. B) Quantification of normalized p-eIF2α following training (n=4 per time point). C) Diagram of the experimental protocol. Sal003 (40 μM) or Vehicle was infused into the hippocampus immediately after the strong training protocol and dorsal hippocampi were dissected 30 min after training. D) A representative Western blot showing phosphorylation of hippocampal eIF2α after infusion of Sal003. E) Quantification of normalized p- eIF2α using total eIF2α as a loading control (n=4 for vehicle and Sal003 group). F) Diagram of the experimental protocol. Bilateral infusion of Sal003 into the hippocampus immediately after training reduced long-term contextual fear conditioning (G, p < 0.05) but did not affect auditory fear conditioning (H, p > 0.05), both assessed 24 hr after training. Data (means ± S.E.M) from vehicle (n=7) and Sal003-injected rats (n=9) are represented by open and closed columns or symbols, respectively.