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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Arthritis Rheumatol. 2014 Sep;66(9):2380–2390. doi: 10.1002/art.38724

Figure 2.

Figure 2

The CFA-induced arthritic inflammatory phenotype in the paw was similar between genotypes and ages. Representative H&E hairy skin cross-sections from wild-type mice at 8-weeks (4x magnification). (A) Aged CFA-injected paw (top left). Aged PBS-injected paw (top right). Young CFA-injected paw (bottom left). Young PBS-injected paw (bottom right). Black arrows indicated increase leukocyte infiltration and purple arrows indicate bone deformation in CFA-injected animals. (B) Qualitative paw scores indicate no differences between age groups or genotypes until week 8. (C) Combined histological and qualitative measurements indicated no differences between genotypes in either age group. (D) The average paw area increased in young CFA-injected TRPA1+/+ (***; n = 12) and TRPA1-/- (***; n = 13) mice when compared to young PBS-injected TRPA1+/+ (n = 9) and TRPA1-/- (n = 8) controls, respectively. No differences between genotypes were observed within CFA and PBS treatment groups (left). The average paw area was similar between CFA-injected aged TRPA1+/+ (n = 6) and TRPA1-/- (n = 3) mice when compared to aged PBS-injected TRPA1+/+ (n = 3) and TRPA1-/- (n = 4) controls, respectively. No differences between genotypes were observed within CFA and PBS treatment groups (right). Data reported as mean ± s.e.m.; **** p <0.05, *** p <0.001.