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. Author manuscript; available in PMC: 2014 Aug 29.
Published in final edited form as: Free Radic Biol Med. 2012 Dec 20;57:49–60. doi: 10.1016/j.freeradbiomed.2012.12.004

Fig. 1.

Fig. 1

LC/MS chromatogram (EICs) of POBN adducts formed from cellular COX-catalyzed AA and DGLA peroxidation. HCA-7 colony 29 cells were trypsinized, harvested, and suspended in PBS and treated with POBN and PUFAs as described in Materials and Methods. After a 30-min incubation, the reaction was stopped using ACN (1/1, v/v), and the supernatant was collected, centrifuged, and condensed for LC/MS analysis. (A) EICs of m/z 296, m/z 448, m/z 266 and m/z 548 as formation of POBN/●C5H11, POBN/●C6H13O, POBN/●C14H21O4 and POBN/●C20H34O5 from the C-15 oxygenation of COX-catalyzed AA peroxidation; (B) EICs of m/z 266 and m/z 296 from the formation of POBN/●C5H11, POBN/●C6H13O from C-15 oxygenation of COX-catalyzed DGLA peroxidation; and (C) EICs of m/z 324 and m/z 354 from the formation of POBN/●C7H13O2 and POBN/●C8H15O3 from C-8 oxygenation of COX-catalyzed DGLA peroxidation.