Table 1. Effect of PPA and BA on neurotransmitter related gene expression in PC12 cells.

Gene/fold change	1 mM BA	6 mM BA	1 mM PPA	10 mM PPA
TH	↑2.8±0.21	↓6.4±0.12	↑2.17±0.21	↓2.2±0.35
DBH	↑1.4±0.17	↓14.5±0.89	↑1.2±0.23	↓1.8±0.13
NPY	↑2.78±0.35	↑5.2±0.64	1.02±0.28	↑ 1.3±0.35
ChrA	↑1.8±0.15	↓3.0±0.48	↑1.15±0.11	↓0.85±0.12
PENK	↑4.65±0.39	↑2.46±0.22	↑2.7±0.25	↑1.95±0.25
GTPCH	1.02±0.09	0.98±0.15	↑1.45±0.23	↑2.67±0.27

PC12 cells were treated with 1 mM PPA, 10 mM PPA, 1 mM BA, 6 mM BA or vehicle for 48 hrs. Total RNA samples were isolated from individual petri dishes (n>6 per experimental group). Northern blot analysis was performed as described in Methods section, using probes specific for rat genes, encoding tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), neuropeptide Y (NPY), Chromogranin A (ChrA), pre-proenkephalin (PENK) and GTP cyclohydrolase (GTPCH). The results are presented as mean ± SEM value relative to the mRNA levels in control, vehicle treated cells. 18S rRNA was used as a house keeping gene for normalization of data.