Figure 5. 5-HT_1B/1D receptors-induced regulation of TG2/NF-κB signaling might mediate proliferation/invasion-promoting pathways.

(A) 5-HT_1B and 5-HT_1D receptors regulate TG2 and NF-κB expression. PANC-1 cells were treated with indicated siRNAs as described above. (B) siRNA-mediated TG2 knockdown induced downstream molecular effects similar to that observed after knockdown of 5-HT_1B and 5-HT_1D receptors expression. Cells were transfected with 50 nM of TG2 siRNA or control siRNA, and cell lysates were subjected to Western blot analysis. β-actin was used as loading control. (C) Inhibition of constitutive activation of NF-κB inhibits the key signaling promoting proliferation/invasion and decreases the mesenchymal markers, Fibronectin and α-SMA. Cells were treated with indicated concentration of NF-κB activation Inhibitor II, JSH-23, for 24 h, and cell lysates were subjected to Western blot analysis. Tubulin was used as loading control. Three independent experiments were performed with similar results, and representative data is shown.