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. 2014 Aug 29;9(8):e105502. doi: 10.1371/journal.pone.0105502

Figure 2. Induction of nCRT oligomerization in vitro.

Figure 2

A sample of nCRT purified from murine livers was run on 10% SDS-page gel and analyzed by Western blotting using polyclonal rabbit Abs against CRT (A). Samples of nCRT were incubated for 3 hrs under pH 7.4/37°C (Lane 1), or pH 7.4/42°C (Lane 2), or pH 5.0/37°C (Lane 3), or pH 5.0/42°C (Lane 4), or “pH 5.0/42°C” group was maintained for another 3 hrs in conditions of pH 7.4/37°C (Lane 5) prior to native-page gel analysis followed Western blotting (B). The results are representatives of three independent experiments.