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. 2014 Aug 29;9(8):e104948. doi: 10.1371/journal.pone.0104948

Figure 6. Hepatic SCD1 mRNA and protein levels of wild type, STZ and KKAy mice.

Figure 6

The C16:1/C16:0, C18:1/C18:0 (A) and C18:0/C16:0 (B) ratios were calculated, based on data obtained from the serum fatty acid analysis (line 1:WSC, line 2:WLC, line 3:WSR, line 4:KSC, line 5:KLC, line 6:KSR). SCD1 (C) and Elovl6 (D) mRNA levels of all murine models were analyzed by quantitative real time PCR (white bar: mice fed the SC diet, gray bar: mice fed the LC diet, black bar: mice fed the SR diet). Hepatic SCD1 protein levels of wild type (E), STZ (F) and KKAy (G) mice were analyzed by western blotting. In the middle panels, representative data (two samples for each group) are presented. In the lower panels, each column shows the mean ± S.E. obtained from 6 mice (white bar: mice fed the SC diet, gray bar: mice fed the LC diet, black bar: mice fed the SR diet). Upper panels show the internal control using anti-β actin antibody. *p<0.05 (vs corresponding mice fed the SC diet). **p<0.05 (vs corresponding mice fed the SR diet).