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atp11Δ, atp12Δ, vps36, BY4741(wt) and tim18Δ (positive control) (DUNN and JENSEN 2003) strains were streaked out onto YPD plates containing 25 µg/ml ethidium bromide and allowed to grow for 3 days at 30°C to induce loss of mtDNA. To identify strains killed by the EtBr treatment, cells from YPD-EtBr plates were streaked to YPD plates lacking EtBr, and allowed to grow at 30°C for 3 days.
