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. 2014 Aug 29;9(8):e105953. doi: 10.1371/journal.pone.0105953

Figure 3. R-825 reduced iron uptake and stimulated the expressions of PiaA and PiuA in S. pneumoniae.

Figure 3

(A) The cellular iron and ruthenium contents of the wild-type S. pneumoniae with and without R-825 treatment. (B) The increase of mRNA expression levels of piaA, piuA and pitA in the presence of 15 µM R-825, as compared with their expressions in the absence of R-825. Data were normalized with housekeeping gene 16S rRNA (mean ± SEM, n  =  3, **p < 0.01, *p < 0.05 versus the untreated control). (C) The comparison of the protein expression levels in the bacterium with and without R-825 treatment. Whole cell proteins were used as loading control for the Western blotting.