Table 1. Forward and reverse primers used in reverse transcription polymerase chain reaction.

Rat gene	Primer sequence (5′-3′)	Annealing temperature (°C)	Product size (bp)
GAPDH	5′-CAGTGCCAGCCTCGTCTCAT-3′ 5′-AGGGGCCATCCACAGTCTTC-3′	58	595
TNF-α	5′-CACCACGCTCTTCTGTCTACTG-3′ 5′-AGATAAGGTACAGCCCATCTGC-3′	57	281
IL-6	5′-CAAGAGACTTCCAGCCAGTTG-3′ 5′-GAAACGGAACTCCAGAAGACC-3′	55	350

TNF-α: tumor necrosis factor-α; IL: interleukin.