Table 2. Specificity, sensitivity and kappa index of MSP-PCR fingerprinting using the primer (GTG)5 in comparison with rDNA sequencing as the gold standard for the two datasets (“lower diversity” and “higher diversity”), using two ranges of band sizes: 200–3500bp or 200–900 bp.
Dataset | Range of band size | MSP-PCR fingerprinting | Gold standard (sequencing) | Kappa index | |
(GTG5) | |||||
“Lower diversity” | 200–3500bp | Specificity | 23.30% | 100.00% | 0.177 |
Sensitivity | 100.00% | 41.70% | |||
200–900bp | Specificity | 15.20% | 100.00% | 0.169 | |
Sensitivity | 100.00% | 60.30% | |||
“Higher diversity” | 200–3500bp | Specificity | 33.70% | 97.10% | 0.201 |
Sensitivity | 97.40% | 35.60% | |||
200–900bp | Specificity | 20.20% | 95.50% | 0.124 | |
Sensitivity | 97.70% | 36.40% |