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. 2014 Aug 29;9(8):e105792. doi: 10.1371/journal.pone.0105792

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© 2014 Leung et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Phosphorylation of ERK, AKT, p70S6K and rpS6 in (A) MCF-7, (B) T47D, (C) SKBr3 and (D) MDA-MB-231 cell lines treated with indicated concentration of trametinib for 1 or 24 h. Immunoblots for phospho-proteins and their respective total proteins are indicated below. Tubulin is the loading control.