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. 2014 Aug 29;9(8):e105883. doi: 10.1371/journal.pone.0105883

Figure 4. CCR1 activity on hematopoietic and non-hematopoietic cells modulates the writhing response.

Figure 4

(A) WT leukocytes were isolated from the peritoneal cavity 4 hours after thioglycollate injection. 2.9 million cells were injected into WT and CCR1−/− mice and immediately tested in the acetic acid-induced writhing model (n = 5–10). (B) WT and Pep Boy mice were tested in the acetic acid-induced writhing model (n = 10). (C) Bone marrow transplants were performed to generate chimeric mice using WT (CD45.2), CCR1−/− (CD45.2), and Pep Boy (CD45.1) mice. (D) Engraftment of bone marrow was assessed after 10 weeks by measuring CD45.1 versus CD45.2 expression. (E) Chimeric mice were tested in the acetic acid-induced writhing model (n = 5–12). P<0.05 and **P<0.01.