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. 2014 Aug 29;9(8):e106048. doi: 10.1371/journal.pone.0106048

Figure 6. Weak iNOS immunolabeling of ramified microglia in the developing quail retina during the first post-hatching week.

Figure 6

Representative confocal images of QH1 (green) and anti-iNOS (red) double-immunolabeled microglial cells in a whole-mounted retina (WM, A-C) and retinal cross sections (CS, D) from 4-day-old quails (P4). Hoechst staining was used to visualize nuclei (blue) in the retinal cross section. Microglial cells at this developmental stage are mainly located in the nerve fiber layer (NFL, A), inner plexiform layer (IPL, B), and outer plexiform layer (OPL, C) and show a more profuse ramification and a similar anti-iNOS immunostaining in comparison to ramifying microglial cells in retinas from embryos at 14 days of incubation. During the first post-hatching week, some microglial cells can be seen in the inner nuclear layer (INL), apparently traversing this layer towards the OPL (D). GCL: ganglion cell layer. Scale bar, 50 µm for A-C; 65 µm for D.