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. 2014 Aug 29;9(8):e106188. doi: 10.1371/journal.pone.0106188

Figure 1. PPVO sensing requires endosomal maturation in pDC but not in cDC.

Figure 1

BMDC were generated in the presence of Flt3-ligand and pDC and cDC purified by FACS as described in material and methods. Purified pDC and cDC were stimulated in triplicates as indicated for 24 h in the absence or presence of chloroquine and supernatants were analysed for the indicated cytokines. Concentrations of cytokines are presented as mean +/− SEM. One representative experiment of three is presented for cytokine data. Remaining cells were stained and analysed for the expression of co-stimulatory CD86 presented as mean +/− SEM of the median fluorescence intensity. Pooled data of two experiments are shown for CD86 expression. Statistically significant differences between stimulations in the absence and presence of chloroquine were determined by 2-way ANOVA and Bonferroni post-test and are indicated by asterisks.