Figure 2. PPVO-induced pDC activation is blocked by TLR9-specific iCpG-ODN.

Purified BM-pDC were stimulated in triplicates as indicated for 24 h in the absence or presence of iCpG-ODN (iCpG) and supernatants were analysed for the indicated cytokines. Concentrations of cytokines are presented as mean +/− SEM. One representative experiment of three is presented for cytokine data. Remaining cells were stained and analysed for the expression of co-stimulatory CD86 presented as the mean +/− SEM of the median fluorescence intensity. Pooled data of three experiments are shown for CD86 expression. Statistically significant differences between stimulations in the absence and presence of iCpG-ODN (5 µM) were determined by 2-way ANOVA and Bonferroni post-test and are indicated by asterisks.