|
Plasmids
|
| pK18mobsacB |
Small mobilizable suicide vector, sucrose-sensitive (sacB)/KanR
|
[78]
|
| pHL1 |
Destination vector for transcriptional fusion using LR reaction,carrying gfp/TetR CamR
|
This study |
| pBS181 |
phrpJ::iucD expresses GUS underT3SS inducing conditions/TetR KanR
|
This study |
| pCPP6424 |
pENTR/SD/D-TOPO::PSPTO_5633; Entry vector withthe coding region of PSPTO_5633 lacking the stop codon/KanR
|
This study |
| pCPP5371 |
Cya Gateway destination vector/GenR CamR
|
[43]
|
| pCPP6413 |
pCPP5371::PSPTO_5633/GenR
|
This study |
| pCPP5388 |
pCPP5371::AvrPto/GenR
|
This study |
| pBS62 |
pENTR/D with DC3000 genomic coordinates1542594.1542860; contains hrpJ promoter/KanR
|
This study |
| pBS180 |
iucD reporter promoter trap destination vector; Gatewaycassette cloned upstream of iucD/TetR KanR CamR
|
This study |
| pBS59 |
lux operon reporter promoter trap destination vector;Gateway cassette cloned upstreamof lux operon/TetR KanR CamR
|
[40]
|
| pTGS |
Source of gfp mut2/TetR
|
[41]
|
| pBS12 |
gfp reporter promoter trap vector/TetR
|
This study |
| HLN068 |
pENTR/SD/D-TOPO::PPSPTO_0871; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN069 |
pENTR/SD/D-TOPO::PPSPTO_0816; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN100 |
pENTR/SD/D-TOPO::PPSPTO_2130; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN101 |
pENTR/SD/D-TOPO::PPSPTO_4750; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN102 |
pENTR/SD/D-TOPO::PPSPTO_5053; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN103 |
pENTR/SD/D-TOPO::PPSPTO_5618; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN105 |
pENTR/SD/D-TOPO::PPSPTO_1843; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN106 |
pENTR/SD/D-TOPO::PPSPTO_3481; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN107 |
pENTR/SD/D-TOPO::PPSPTO_3721; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN108 |
pENTR/SD/D-TOPO::PPSPTO_4340; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN206 |
pENTR/SD/D-TOPO::PPSPTO_5633; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN207 |
pENTR/SD/D-TOPO::PPSPTO_0371; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN208 |
pENTR/SD/D-TOPO::PPSPTO_1645; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN210 |
pENTR/SD/D-TOPO::PPSPTO_2691; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN212 |
pENTR/SD/D-TOPO::PPSPTO_2696; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN214 |
pENTR/SD/D-TOPO::PPSPTO_3331; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN215 |
pENTR/SD/D-TOPO::PPSPTO_3948_9; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN216 |
pENTR/SD/D-TOPO::PPSPTO_4699; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN217 |
pENTR/SD/D-TOPO::PPSPTO_4721; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN218 |
pENTR/SD/D-TOPO::PPSPTO_4955; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN250 |
pENTR/SD/D-TOPO::PPSPTO_5240; Entry vector containingcandidate hrp promoter upstream from noted gene/KanR
|
This study |
| HLN072 |
pHL1::PPSPTO_0871::gfp; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN162 |
pHL1::PPSPTO_2130::gfp; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN163 |
pHL1::PPSPTO_4750::gfp; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN164 |
pHL1::PPSPTO_5053::gfp; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN165 |
pHL1::PPSPTO_5618::gfp; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN167 |
pHL1::PPSPTO_1843; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN168 |
pHL1::PPSPTO_3481; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN169 |
pHL1::PPSPTO_3721; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN170 |
pHL1::PPSPTO_4340; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN219 |
pHL1::PPSPTO_5633; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN220 |
pHL1::PPSPTO_0371; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN221 |
pHL1::PPSPTO_1645; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN223 |
pHL1::PPSPTO_2691; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN225 |
pHL1::PPSPTO_2696; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN227 |
pHL1::PPSPTO_3331; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN228 |
pHL1::PPSPTO_3948_9; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN229 |
pHL1::PPSPTO_4699; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN230 |
pHL1::PPSPTO_4721; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN231 |
pHL1::PPSPTO_4955; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN253 |
pHL1::PPSPTO_0816; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
| HLN254 |
pHL1::PPSPTO_5240; Expression vector containingcandidate hrp promoter fused to gfp reporter/TetR
|
This study |
|
Escherichia coli
|
| TOP10 |
Φ80lacZΔM15 ΔlacX74
|
Invitrogen |
|
Pseudomonas syringae pv. tomato |
| DC3000 |
DC3000/RifR
|
[79]
|
| CUCPB5460 |
DC3000 with hopQ1-1 deleted (ΔhopQ1-1)/RifR
|
[3]
|
| UNL-134–1 |
DC3000 with hrpL deleted (ΔhrpL)/RifR SpcR
|
[23]
|
| DC3000pBS181 |
DC3000 carrying phrpJ::iucD; expresses GUSunder T3SS inducing conditions/TetR KanR RifR
|
This study |
|
hrpL-
FLAGpBS181 |
HLN090 carrying phrpJ::iucD; expresses GUSunder T3SS inducing conditions/TetR KanR RifR
|
This study |
| HLN090 |
DC3000 with hrpL tagged byFLAG at its C-terminus (hrpL-FLAG)/RifR
|
This study |
| HLN010 |
DC3000 with PSPTO_5633 on chromosomedeleted; remaining another copy on plasmid B/RifR
|
This study |
| HLN009 |
DC3000 with hopQ1-1 and PSPTO_5633 onchromosome deleted/RifR
|
This study |
| HLN330 |
DC3000 with PSPTO_5633 on plasmidand chromosome deleted/RifR
|
This study |
| HLN012 |
DC3000 with PSPTO_0371 deleted/RifR
|
This study |
| HLN011 |
DC3000 with hopQ1-1 and PSPTO_0371 deleted/RifR
|
This study |
| HLN014 |
DC3000 with PSPTO_2130 deleted/RifR
|
This study |
| HLN013 |
DC3000 with hopQ1-1 and PSPTO_2130 deleted/RifR
|
This study |
| HLN016 |
DC3000 with PSPTO_2691 deleted/RifR
|
This study |
| HLN015 |
DC3000 with hopQ1-1 and PSPTO_2691 deleted/RifR
|
This study |
| HLN018 |
DC3000 with PSPTO_2696 deleted/RifR
|
This study |
| HLN017 |
DC3000 with hopQ1-1 and PSPTO_2696 deleted/RifR
|
This study |
| HLN020 |
DC3000 with PSPTO_3331 deleted/RifR
|
This study |
| HLN019 |
DC3000 with hopQ1-1 and PSPTO_3331 deleted/RifR
|
This study |
| HLN182 |
DC3000 with PSPTO_5240 deleted/RifR
|
This study |
| HLN183 |
DC3000 with hopQ1-1 and PSPTO_5240 deleted/RifR
|
This study |
| HLN190 |
ΔpvsA carrying pHL1::PPSPTO_2130::gfp; gfp under control ofpromoter upstream of PSPTO_2130/RifR TetR KanR
|
This study |
| HLN191 |
ΔpvsA carrying pHL1::PPSPTO_4750::gfp; gfp under control ofpromoter antisense of PSPTO_4750/RifR TetR KanR
|
This study |
| HLN192 |
ΔpvsA carrying pHL1::PPSPTO_5053::gfp; gfp under control ofpromoter upstream of PSPTO_5053/RifR TetR KanR
|
This study |
| HLN193 |
ΔpvsA carrying pHL1::PPSPTO_5618::gfp; gfp under control ofpromoter upstream of PSPTO_5618/RifR TetR KanR
|
This study |
| HLN195 |
ΔpvsA carrying pHL1::PPSPTO_1843::gfp; gfp under control ofpromoter upstream of PSPTO_1843/RifR TetR KanR
|
This study |
| HLN196 |
ΔpvsA carrying pHL1::PPSPTO_3481::gfp; gfp under control ofpromoter upstream of PSPTO_3481/RifR TetR KanR
|
This study |
| HLN197 |
ΔpvsA carrying pHL1::PPSPTO_3720::gfp; gfp under control ofpromoter upstream of PSPTO_3720/RifR TetR KanR
|
This study |
| HLN198 |
ΔpvsA carrying pHL1::PPSPTO_4340::gfp; gfp under control ofpromoter upstream of PSPTO_4340/RifR TetR KanR
|
This study |
| HLN232 |
ΔpvsA carrying pHL1::PPSPTO_5633::gfp; gfp under control ofpromoter upstream of PSPTO_5633/RifR TetR KanR
|
This study |
| HLN233 |
ΔpvsA carrying pHL1::PPSPTO_0371::gfp; gfp under control ofpromoter upstream of PSPTO_0371/RifR TetR KanR
|
This study |
| HLN234 |
ΔpvsA carrying pHL1::PPSPTO_1645::gfp; gfp under control ofpromoter upstream of PSPTO_1645/RifR TetR KanR
|
This study |
| HLN237 |
ΔpvsA carrying pHL1::PPSPTO_2691::gfp; gfp under control ofpromoter upstream of PSPTO_2691/RifR TetR KanR
|
This study |
| HLN238 |
ΔpvsA carrying pHL1::PPSPTO_2696::gfp; gfp under control ofpromoter upstream of PSPTO_2696/RifR TetR KanR
|
This study |
| HLN240 |
ΔpvsA carrying pHL1::PPSPTO_3331::gfp; gfp under control ofpromoter upstream of PSPTO_3331/RifR TetR KanR
|
This study |
| HLN241 |
ΔpvsA carrying pHL1::PPSPTO_3948_3949::gfp; gfp under controlof a hrp promoter in the middlePSPTO_3948 and PSPTO_3949/RifR TetR KanR
|
This study |
| HLN242 |
ΔpvsA carrying pHL1::PPSPTO_4699::gfp; gfp under control ofpromoter upstream of PSPTO_4699/RifR TetR KanR
|
This study |
| HLN243 |
ΔpvsA carrying pHL1::PPSPTO_4721::gfp; gfp under control ofpromoter upstream of PSPTO_4721/RifR TetR KanR
|
This study |
| HLN244 |
ΔpvsA carrying pHL1::PPSPTO_4955::gfp; gfp under control ofpromoter upstream of PSPTO_4955/RifR TetR KanR
|
This study |
| HLN263 |
ΔpvsA carrying pHL1::PPSPTO_0816::gfp; gfp under control of apromoter upstream of of PSPTO_0816/RifR TetR KanR
|
This study |
| HLN264 |
ΔpvsA carrying pHL1::PPSPTO_0871::gfp; gfp under control of apromoter upstream of PSPTO_0871/RifR TetR KanR
|
This study |
| HLN265 |
ΔpvsA carrying pHL1::PPSPTO_5240::gfp; gfp under control of apromoter upstream of PSPTO_5240/RifR TetR KanR
|
This study |
| HLN245 |
ΔpvsA carrying pHL1::Pφ::gfp;; empty plasmid asnegative control in gfp assay/RifR TetR KanR
|
This study |
| PS167 |
DC3000 ΔpvsA/RifR KanR
|
[42]
|
| DC3000T3SS- |
CUCPB5113, DC3000ΔhrcQ-U/RifR SpcR
|
[80]
|
| DC3000T2SS- |
DC3000ΔgspD/SpcR
|
[81]
|
| HLN397 |
DC3000 T3SS- carrying PSPTO_5633-Cya fusion (pCPP6413)/RifR GenR
|
This study |
| HLN078 |
DC3000 carrying PSPTO_5633-Cyafusion (pCPP6413)/RifR GenR
|
This study |
| HLN396 |
DC3000 T2SS- carrying PSPTO_5633-Cya fusion (pCPP6413)/RifR GenR
|
This study |
| HLN079 |
DC3000 carrying AvrPto-Cya fusion(pCPP5388)/RifR GenR
|
This study |
| HLN074 |
DC3000 T3SS- carrying AvrPto-Cyafusion (pCPP5388)/RifR GenR
|
This study |
