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. 2014 Aug 29;9(8):e106129. doi: 10.1371/journal.pone.0106129

Figure 7. Effect of wild-type wnk1a and wnk1b mRNA injection on flk1, wnk1a and wnk1b morphants.

Figure 7

(A) Co-injection of wnk1a, wnk1b or both wnk1a and wnk1b mRNA rescues flk1 morphants. (B) Co-injection of wnk1a mRNA rescues the ISV defect caused by the wnk1a upstream MO. (C) Co-injection of wnk1b mRNA rescues the ISV defect caused by the wnk1b upstream MO. (D) Injection of rat Wnk1(1–449) rescues flk1 morphants. Experiments were performed at least three times, and the number of embryos analyzed is shown in the bar graph. Red indicates that the ISVs grew to full length, orange indicates that the ISVs were 75% of the normal length, yellow indicates 50%, green indicates 25%, and light blue indicates no ISVs were observed in the embryos. The differences between treatments were assessed using a two-tailed Student’s t-test. Significant differences between the morphants and controls are indicated (*, P<0.05; **, P<0.01; and ***, P<0.001); significant differences between co-injection of RNA with morpholino and morpholino alone are also indicated (#, P<0.05; ##, P<0.01; and ###, P<0.001).