Figure 2. In vivo hydrogen consumption in S. denitrificans DSM-1251.

S. denitrificans DSM-1251 cells were grown anaerobically in DSMZ medium 113 either with thiosulfate (18.8 mM) or without thiosulfate. On the first day of inoculation, the headspace of each sample was exchanged with H₂/CO₂ (80%/20%). The H₂ concentration in the headspace was measured with gas chromatography. A control experiment was set up to exclude hydrogen leakage from the vessels. Medium = control, medium without inoculated S. denitrificans DSM-1251; S. den. = inoculation with 0.5 mL of S. denitrificans DSM-1251 pre-culture at day 1. Error bars of “Medium” and “S. den.” indicate the standard deviations from three independent experiments.