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. 2014 Aug 29;9(8):e106218. doi: 10.1371/journal.pone.0106218

Figure 5. Hydrogen uptake activity of the native and recombinant hydrogenase (HydB) from S. denitrificans.

Figure 5

Hydrogen uptake activity was measured in the membrane fractions of S. denitrificans grown without and with thiosulfate (18.8 mM) and for the recombinant version of the large subunit (HydB). The large subunit of the periplasmic hydrogenase (hydB) was cloned into the vector pBBR1MCS-2 and expressed heterologously in S. oneidensis ΔhyaB. The empty vector in S. oneidensis ΔhyaB was used as negative control. Error bars denote standard deviations from three independent measurements.