Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1974 Jul;10(1):54–59. doi: 10.1128/iai.10.1.54-59.1974

Further Characterization of Staphylococcal Gamma-Hemolysin

Anthony G Taylor a,1, Alan W Bernheimer a
PMCID: PMC414956  PMID: 4601977

Abstract

It was confirmed that staphylococcal gamma-hemolysin is composed of two separate proteins (gamma-lysin components I and II) which act synergistically. The molecular weights of the two components, determined by gel filtration, are 29,000 and 26,000, respectively, and their isoelectric points, determined by isoelectric focusing, are at pH 9.8 and 9.9. Both components are susceptible to the action of Pronase and subtilisin. A wide range of lipids, some in minute amounts, are capable of inhibiting the hemolytic activity of gamma-lysin.

Full text

PDF
54

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. doi: 10.1042/bj0910222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Colley C. M., Zwaal R. F., Roelofsen B., van Deenen L. L. Lytic and non-lytic degradation of phospholipids in mammalian erythrocytes by pure phospholipases. Biochim Biophys Acta. 1973 Apr 25;307(1):74–82. doi: 10.1016/0005-2736(73)90026-6. [DOI] [PubMed] [Google Scholar]
  3. Doery H. M., Magnusson B. J., Gulasekharam J., Pearson J. E. The properties of phospholipase enzymes in staphylococcal toxins. J Gen Microbiol. 1965 Aug;40(2):283–296. doi: 10.1099/00221287-40-2-283. [DOI] [PubMed] [Google Scholar]
  4. GLADSTONE G. P., VAN HEYNINGEN W. E. Staphylococcal leucocidins. Br J Exp Pathol. 1957 Apr;38(2):123–137. [PMC free article] [PubMed] [Google Scholar]
  5. Guyonnet F., Plommet M. Hémolysine gamma de staphylococcus aureus: purification et propriétés. Ann Inst Pasteur (Paris) 1970 Jan;118(1):19–33. [PubMed] [Google Scholar]
  6. Guyonnet F. Toxicité de la toxine gamma de Staphylococcus aureus pour la souris. Ann Rech Vet. 1970;1(2):155–160. [PubMed] [Google Scholar]
  7. HOWARD J. G., WALLACE K. R., WRIGHT G. P. The inhibitory effects of cholesterol and related sterols on haemolysis by streptolysin O. Br J Exp Pathol. 1953 Apr;34(2):174–180. [PMC free article] [PubMed] [Google Scholar]
  8. JACKSON A. W., LITTLE R. M. Staphylococcal toxins. II. Factors affecting hemolysis by delta-lysin. Can J Microbiol. 1958 Oct;4(5):435–444. doi: 10.1139/m58-046. [DOI] [PubMed] [Google Scholar]
  9. Kapral F. A. Inhibition of Staphylococcus aureus delta hemolysin by phospholipids. Proc Soc Exp Biol Med. 1972 Nov;141(2):519–521. doi: 10.3181/00379727-141-36812. [DOI] [PubMed] [Google Scholar]
  10. Kreger A. S., Kim K. S., Zaboretzky F., Bernheimer A. W. Purification and properties of staphylococcal delta hemolysin. Infect Immun. 1971 Mar;3(3):449–465. doi: 10.1128/iai.3.3.449-465.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Möllby R., Wadström T. Separation of Gamma Hemolysin from Staphylococcus aureus Smith 5R. Infect Immun. 1971 Apr;3(4):633–635. doi: 10.1128/iai.3.4.633-635.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SMITH D. D. Experimental staphylococcal infection in mice. J Pathol Bacteriol. 1962 Oct;84:359–365. doi: 10.1002/path.1700840210. [DOI] [PubMed] [Google Scholar]
  13. Taylor A. G. A ribonuclease of group A streptococci. J Gen Microbiol. 1971 Feb;65(2):193–200. doi: 10.1099/00221287-65-2-193. [DOI] [PubMed] [Google Scholar]
  14. Taylor A. G., Plommet M. Anti-gamma haemolysin as a diagnostic test in staphylococcal osteomyelitis. J Clin Pathol. 1973 Jun;26(6):409–412. doi: 10.1136/jcp.26.6.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vesterberg O., Wadström T., Vesterberg K., Svensson H., Malmgren B. Studies on extracellular PROTEINS FROM Staphylococcus aureus. I. Separation and characterization of enzymes and toxins by isoelectric focusing. Biochim Biophys Acta. 1967 Apr 11;133(3):435–445. doi: 10.1016/0005-2795(67)90547-8. [DOI] [PubMed] [Google Scholar]
  16. Wadström T., Möllby R. Some biological properties of purified staphylococcal haemolysins. Toxicon. 1972 Aug;10(5):511–519. doi: 10.1016/0041-0101(72)90177-8. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES