Figure 6. ATP release is reduced by Panx1 channel blockade and enhanced by low divalent cation solution.

(A) Mechanical stimulation imposed by rinsing the cells with bathing solution induced ATP release from TRT-HU1 cells that was significantly higher than that from non-stimulated cells when measured in the presence or absence of the Panx1 channel blocker mefloquine (MFQ, 100 nM; n = 4 each, *P<0.05 and **P<0.01, by paired t-test.). (B) Normalized ATP release with respect to basal values, however, was significantly lower in MFQ-treated compared to untreated cells (N = 4, ^$ P<0.05 by Mann-Whitney U test). (C) Exposure to low divalent cation solution (LDPBS), a condition known to enhance P2X₇R activation, significantly increased ATP release from TRT-HU1 cells. All data represent mean ± SEM (N = 4 each, *P<0.05 by Student’s t-test).