Figure 9. Dose-response plot of Y. pestis MEP synthase with the top five rationally designed inhibitors; compounds A) 13, B) 15, C) 16, D) 42 and E) 48.

Assays were performed by combining the enzyme with 150 µM NADPH, followed by addition of the inhibitor. After five minute incubation at 37°C, substrate was added to initiate the reaction. The R² value for each plot is indicated. The enzymatic activity is relative to an uninhibited control. All assays were performed in duplicate.