Figure 10. Dose-response plot of the Y. pestis MEP synthase when preincubated with the inhibitor.

Assays were performed by combining the enzyme with either A) compound 15 or B) compound 16 and preincubating at 37°C for 10 min before addition of NADPH and DXP. All assays were performed in duplicate. Activity of the enzyme is relative to an uninhibited control.