Figure 14. Screening of a natural product library.
The Y. pestis MEP synthase was assayed in the presence of ca. 75 µg/mL of the indicated extract. Residual activity is relative to the assay performed with vehicle alone (DMSO). Assays with fosmidomycin and FR900098 were performed with 100 µM inhibitor. All assays were performed in duplicate. Of those compounds tested, four inhibit the enzymatic activity by >75%, including e5, e18, e29, and e74. The activity of e29 is comparable to that of fosmidomycin.
