Table 1. Assembly results of sugarcane transcriptome using trinity software.

Length range	Unigene	Merge_Unigene
200 bp–300 bp	28,473 (30.85%)	27,684 (27.73%)
300 bp–500 bp	24,852 (26.93%)	24,795 (24.84%)
500 bp–1,000 bp	18,488 (20.03%)	25,254 (25.30%)
1,000 bp–2,000 bp	12,792 (13.86%)	14,383 (14.41%)
2,000+bp	7,692 (8.33%)	7,708 (7.72%)
Total number	92,297	99,824
Total length	70,251,430 bp	77,293,229 bp
N50 length	1,300 bp	1,143 bp
Mean length	761.1453 bp	774.2950 bp

Notes: N50 length is an indicator of measuring assembly effect, which is calculated by the accumulated length of the assembled fragments from long to short. When the sum is greater than or equal to 50% of the total length, the final accumulated fragment length is the N50 value. Mean length = for the average assembly length.