Inhibitors of PKG interfere with heparin responses in primary VSMC. (Panel A) Porcine VSMC were treated identically to A7r5 cells with PDGF stimulation for 10 min. Heparin and inhibitor treatments and staining were identical to that in Figure 5. (Panel B) Porcine VSMC were treated identically and active ERK analyzed by western blotting. Lanes 1 – untreated control, 2 – PDGF 10 min, 3 – heparin only, 4 – heparin and PDGF, 5 – heparin and Rp8-cCPT-cGMPS, 6 – heparin, Rp8-cCPT-cGMPS and PDGF, 7 – Rp8-cCPT-cGMPS and PDGF.