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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: J Cell Physiol. 2014 Dec;229(12):1952–1962. doi: 10.1002/jcp.24645

Figure 3. Real-time PCR validation of genes identified by microarray analysis.

Figure 3

(A) hPNPaseold-35–stable knockdown HO-1cells, (B) hPNPaseold-35–stable knockdown WM35 cells, (C) HeLa cells transiently transfected with siRNA against hPNPaseold-35 and (D) HO-1 cells transfected with pcDNA3.1-hPNPaseold-35. Error bars represent mean ± S.E. of three independent experiments done in triplicate. Statistical significance was determined by a one-way analysis of variance followed by Dunnett’s multiple comparison test and a two-tailed student’s t-test, *P<0.05, **P<0.01, ***P<0.001.