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. 2014 Jul 31;3:398. doi: 10.1186/2193-1801-3-398

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© Utsumi et al.; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Selective cytotoxic effect of NEAPP-AM on TOV21G cells compared with OHFC and HPMC. Cell viability of TOV21G, OHFC, and HPMC after being treated with NEAPP-AM-8 was measured by the cell viability assay. Cells were seeded in collagen-coated 96-well plates and incubated for 24 hrs, the culture medium was replaced with NEAPP-AM, and the cells were incubated for another 24 hrs. The cell proliferation rate was evaluated relative to the control with the cell viability assay. *P < 0.05, **P < 0.01 versus TOV21G treated with NEAPP-AM-8.