Figure 9.

Peroxygenase activity is a prerequisite for the protective function of RD20. A, Seedlings of control lines and lines overexpressing active or catalytically impaired enzyme were grown on NAA. Bars = 1 mm. B, The degree of stress of such lines was estimated by measuring their anthocyanin contents after 1 week of growth in the presence of ROS inducers (n = 10 in four independent experiments; **P < 0.01 and *P < 0.05 when analyzed by Student’s t test). fw, Fresh weight. C, qRT-PCR was used to access the expression of RD20 and ERF1 in leaves of wild-type plants and ctr1-1 and ein2-1 ethylene mutants treated with Paraquat. The results shown represent means ± se of at least four biological replicates (*P ≤ 0.05 and **P < 0.01 when analyzed by the Student-Newman-Keuls test).