Figure 4.

Phylogenetic tree of TNL subclass NBS genes based on conserved NBS domain sequences. A total of 623 nTNL subclass NBS sequences were used: 197 sequences from M. truncatula (shown in red), 95 from pigeon pea (blue), 88 from common bean (orange), 144 from soybean (green), and 99 from Arabidopsis (At; black). Two nTNL sequences from Arabidopsis (purple) were used as an outgroup. The reconstructed TNL phylogeny is divided into eight TNL-rosid NBS gene families and 55 TNL-legume NBS gene families (see “Materials and Methods”). The presence of Arabidopsis or legume sequences in nTNL-rosid families is indicated with check marks. The scale bar represents the number of nucleotide substitutions per site. The support values (SH-aLRT values) for basal nodes are indicated.